Plasmid
Part:BBa_K3978047:Design
Designed by: Minqi Hu Group: iGEM21_HiZJU-China (2021-10-21)
reget-plasmid-mazF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 112
Illegal XbaI site found at 30
Illegal PstI site found at 131 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 112
Illegal PstI site found at 131
Illegal NotI site found at 149 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 112
Illegal BglII site found at 305
Illegal BamHI site found at 106
Illegal XhoI site found at 354 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 112
Illegal XbaI site found at 30
Illegal PstI site found at 131 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 112
Illegal XbaI site found at 30
Illegal PstI site found at 131
Illegal NgoMIV site found at 324
Illegal NgoMIV site found at 671
Illegal NgoMIV site found at 5348 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1256
Illegal SapI.rc site found at 2916
Design Notes
We have no special changes has been done to the sequence.
Source
This part is designed by ourselves.